Extended Data Fig. 10: 3D super-resolution reconstructions of immunofluorescence-labeled elastic fibers in developing cartilage in 20-μm-thick tissue slices using INSPR and in vitro methods in biplane setup.
From: Three-dimensional nanoscopy of whole cells and tissues with in situ point spread function retrieval
a, x-y overview of the reconstructed volume of immunofluorescence-labeled elastic fibers in developing cartilage using INSPR. b, Diffraction-limited image of (a). c–k, x-z slices along the white, magenta, and yellow dashed lines in (a), reconstructed using INSPR (c,f,i), phase retrieval method based on beads on the coverslip with theoretical index mismatch model (PR + IMM, d,g,j), and phase retrieval method based on beads on the coverslip (PR, e,h,k). The integration width of the x-z slices in the y direction is 2 μm.
