Extended Data Fig. 3: Blind reconstruction of 3D training datasets of microtubules (MT0.N1.LD) from the SMLM challenge.
From: Three-dimensional nanoscopy of whole cells and tissues with in situ point spread function retrieval
a,b, x-y and x-z overviews of the microtubules resolved by INSPR from the 3D-Biplane data. c,d, Enlarged x-y and x-z views of the areas as indicated by the magenta and blue boxed regions in (a) and (b), respectively. e,f, Intensity profiles along the y and z directions within the white boxed regions in (c,d), comparing the INSPR resolved profiles (blue solid lines) with the ground truth (red dashed lines). g, x-y views of the provided calibration PSF (3D-Biplane, top rows) and the INSPR retrieved PSF from blinking data (bottom rows). h,i, x-y and x-z overviews of the microtubules resolved by INSPR from the 3D-Astigmatism data. j,k, Enlarged x-y and x-z views of the areas as indicated by the magenta and blue boxed regions in (h) and (i), respectively. l,m, Intensity profiles along the y and z directions within the white boxed regions in (j,k), comparing the INSPR resolved profiles (blue solid lines) with the ground truth (red dashed lines). n, x-y views of the provided calibration PSF (3D-Astigmatism, top rows) and the INSPR retrieved PSF from blinking data (bottom rows). Scale bar in (g,n): 1 µm. Norm.: normalized.
