Extended Data Fig. 1: Schematic of the Nativeomics workflow for identification of ligands from soluble and membrane proteins assemblies. | Nature Methods

Extended Data Fig. 1: Schematic of the Nativeomics workflow for identification of ligands from soluble and membrane proteins assemblies.

From: Combining native and ‘omics’ mass spectrometry to identify endogenous ligands bound to membrane proteins

Extended Data Fig. 1

Successive rounds of MS/MS are applied to identify ligands bound to a, soluble, and b, membrane, protein assemblies. For multi-subunit complexes this can be achieved with or without subunit localisation of ligand binding, which if desired, adds at least one further MS/MS stage, as indicated in the flow schemes. (see Supplementary Table 1).

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