Extended Data Fig. 3: Further examples of bead sorting with two-dimensional gates performed using soRT-FDC.
From: Intelligent image-based deformation-assisted cell sorting with molecular specificity
a, Sorting for deformation together with maximum fluorescence intensity in a mixture of fluorescent and non-fluorescent polyacrylamide beads with different mechanical properties. b, Sorting for brightness and size in a mixture of beads with different diameters, each made of different material (see Extended Data Fig 2c, d). The color map in scatter plots represents event density. The histograms of features presented in the scatter plots are shown on top and on the right of the corresponding scatter plots, the histograms were fit with superpositions of Gaussian functions (solid lines). The gates used for sorting are outlined in green. Percentages on scatter plots indicate the fraction of beads in the sorting gate. The experiments in a and b were repeated independently 1 and 3 times, respectively, with similar results.
