Extended Data Fig. 9: Nuclear enrichment of gene expression in liver tissue.
From: Highly specific multiplexed RNA imaging in tissues with split-FISH
Zoomed in high-resolution image of mouse liver tissue (same dataset as Fig. 3d) to show selected genes highly enriched in the nucleus: Son (red) and Abcc2 (yellow). DAPI image is overlaid in blue. Grayscale background image is the raw image from a single bit. Across the dataset (n = 1, all 49 FOVs, ~7000 cells), we estimated that Son and Abcc2 were enriched 5.6X and 6.4X respectively in the nucleus compared to outside the nucleus (see methods for details on segmentation of DAPI image).
