Extended Data Fig. 8: Two-round Exchange-PAINT alpha-tubulin and vimentin imaging.
From: Up to 100-fold speed-up and multiplexing in optimized DNA-PAINT
Alpha-tubulin and vimentin were labeled with primary and secondary antibodies. a, Overview image shows specific labeling of vimentin and microtubules. b, Zoom-in to the region highlighted in a. Scale bars, 2 µm (a), 500 nm (b). (Integration time: 100 ms, 20 000 frames, R3/4 50 pM, Excitation intensity: 200 W/cm2). The experiment was repeated two times independently with similar results.
