Extended Data Fig. 10: Improved kinetic properties for barcoding.
From: Up to 100-fold speed-up and multiplexing in optimized DNA-PAINT
Concatenation of binding sequences leads to an increased bright time and a decreased dark time which can be used for barcoding based on these kinetic parameters. Potential barcoding capability was demonstrated on single 1×, 3× and 10× R3 binding sites, because the R3 sequence shows distinct separation of bright and dark time values (Extended Data Figs. 2 and 3, Supplementary Fig. 2). The barcoding capability was tested by using a HDBSCAN clustering algorithm. 84.2% of the 3492 analyzed binding sites were classified as either 1×R3 (blue), 3×R3 (green) or 10×R3 (orange). (Integration time: 100 ms, 20 000 frames, 3 nM R3 Cy3B, Excitation intensity: 25 W/cm2, all structures measured in the same sample).
