Extended Data Fig. 1: Imaging of 20-nm grids with 1xR1 and 5xR1 docking sites.
From: Up to 100-fold speed-up and multiplexing in optimized DNA-PAINT
20-nm grids with 1×R1 and 5×R1 docking sites were measured in the same sample. The data represents linked localizations, hence the brightness scales linearly with the number of binding events. a, Overview image. (b and c) 25 selected structures of 1× and 5×R1 DNA origami, respectively. 1×R1 structures show comparable signal intensity to 5×R1 after 5-fold increase of brightness level. Scale bars, 500 nm (a), 100 nm (b, c). (Integration time: 100 ms, 40 000 frames, 500 pM R1 Cy3B, Excitation intensity: 125 W/cm2). The experiment was repeated two times independently with similar results.
