Extended Data Fig. 4: Quantification of sequence orthogonality.
From: Up to 100-fold speed-up and multiplexing in optimized DNA-PAINT
To evaluate the orthogonality of the designed docking sites, the number of binding events of the respective imager sequences (labeled in each graph) in the DNA origami Exchange-PAINT experiment from Supplementary Fig. 4 is quantified for all six structures in the same sample. The violin plots show the distribution of the calculated mean number of binding events detected per structure. The white circle in the violin plots depicts the median number of binding events. A significant number of binding events was exclusively detected for imagers binding to their corresponding docking sites, highlighting exquisite sequence orthogonality, making the sequences applicable for multiplexing experiments. (Number of analyzed origami structures: 5×R1: n = 3515; 5×R2: n = 2436; 7×R3: n = 2619; 7×R4: n = 2502; 5×R5: n = 2962; 5×R6: n = 3381).
