Extended Data Fig. 5: 6-color Exchange-PAINT in 30 minutes image acquisition time.
From: Up to 100-fold speed-up and multiplexing in optimized DNA-PAINT
6 different 20-nm-grid origami structures carrying 6 orthogonal concatenated docking sites were measured sequentially with an imaging time of no longer than 5 minutes per round. Each channel shows clearly resolved and sampled 20-nm-grids and NeNa values below 4 nm (see Supplementary Table 10) in two exemplary field of views. Scale bar: 500 nm. (Integration time: 50 ms, 5 500 frames, Imager concentrations: R1_6nt (5 nM), R2_6nt (2 nM), R3_2 (5 nM), R4_6nt (5 nM), R5 (10 nM), R6_6nt (10 nM), Excitation intensity: 650 W/cm2). The experiment was repeated three times independently with similar results.
