Extended Data Fig. 1: Azide probes.
From: Mid-infrared metabolic imaging with vibrational probes
A single azide band at 2098 cm−1 can be consistently detected on azido-palmitic acid labeled (a) and AHA labeled (b) but not on unlabeled control inside Raw264.7 cells. c, High-definition imaging on single azido-palmitic acid labeled Raw264.7 cells. Pixel size = 0.66 μm. The diffraction-limited resolution is about ~3 μm for the azide band. d, Imaging on C. elegans labeled with azido-palmitic acid. Pixel size = 3.3 μm. Experiments in a-c were repeated five times independently with similar results. Experiments in d were repeated three times independently with similar results. Scale bars, 5 μm in c, 100 μm in d.
