Extended Data Fig. 1: Au(I)-thiolate precipitates formed in the mixtures of HAuCl4 and RSH at classic BSM conditions.
From: Genetically encoded tags for direct synthesis of EM-visible gold nanoparticles in cells
a, Cloudy precipitates formed in the mixture of 2-ME and HAuCl4 at RS−/Au(III) < 2:1 conditions, but no detectable precipitates formed in the mixture at RS−/Au(III) > 2:1 conditions (top row); AuNPs formed (as indicated by the brown colors) in these mixtures at RS−/Au(III) < 2:1 conditions when reduced with NaBH4, while no AuNPs formed (colorless) in those mixtures at RS−/Au(III) > 2:1 conditions (bottom row). b, In D-P and HAuCl4 mixtures: at the state of RS−/Au(III) = 1:1, the solution turned to light brown due to the formation of the unstable (D-P)Au(I) species, but no detectable colors at other conditions (top row); When reduced by NaBH4, the solutions of those RS−/Au(III) < 2:1 conditions turned to brown color, only those RS−/Au(III) > 2:1 cases were still colorless (bottom row). c, Five pairs of experiments revealing the effect of 30 mM D-P for dissolving the cloudy precipitates formed in the mixtures of 0.5 mM HAuCl4 and various concentration of 2-ME. Cloudy precipitates formed in the 0.5 mM HAuCl4 and 10 mM, 20 mM, 40 mM and 80 mM of 2-ME mixtures (the left ones); when added extra 30 mM of D-P into the same mixtures the precipitates were dissolved completely (the right ones). Representative images for (a-c) were selected from n = 3 independent experiments with similar results.
