Extended Data Fig. 7: scNT-seq reveals different stem cell states in mESC cultures.
From: Massively parallel and time-resolved RNA sequencing in single cells with scNT-seq
a. UMAP visualization of 4,633 WT cells (from two biological replicates) colored by different cell-types or cell-states. Feeders are contaminating mouse embryonic fibroblasts. b. UMAP visualization of two biological replicates in (a). c. Violin plots showing total RNA levels (natural log transformation of (TP10K + 1)) of representative marker genes for feeders or specific stem cell states. d. UMAP (same as in (a)) visualization of cells colored by total RNA levels (natural log transformation of (TP10K + 1)) of four representative marker genes. e. Violin plots showing both new and old RNA levels (natural log transformation of (TP10K + 1)) of selected genes across three stem cell states.
