Extended Data Fig. 1: Microtubules and Clatherin in COS cells.

Two-channel data of Microtubules (purple) and Clathrin (green) in COS cells from dataset 5. Scale bar is approximately 1 um. a, A ‘birds- eye’ projection of the two channels in vLUME. b, The same data set from a different point of view closer to the ground to show the 3D nature of the data. To achieve this superposition the first channel has to be opened in vLUME and the color changed. Then the second channel also needs to be opened and changed in color. Subsequently with simple data translation of one of the datasets the two axes need to be overlapped (this task is very simple in VR, manual). The 3D nature of the oblique figure makes it difficult to render a scale bar.