Extended Data Fig. 10: Off-target RNA editing effect for xABE, mxABE, xCBE, mxCBE and REPAIR system.
From: Programmable RNA editing with compact CRISPR–Cas13 systems from uncultivated microbes
a, Transcriptome-wide off-target sites numbers for GFP/mCherry (control), xABE, mxABE, xCBE and mxCBE transfection experiments in HEK293T cells. All values are presented as mean ± s.e.m (n = 3). b, Manhattan plots of transcriptome-wide off-target RNA editing analysis for REPAIR transfection experiments in HEK293T cells (A-to-I editor targeting endogenous SMAD4 RNA). The x and y axis are proportionally enlarged with each Manhattan plot to make the axis legend clear. Non-DR, guide RNA without direct repeats. NT, non-targeting crRNA.
