Extended Data Fig. 4: Construction of spatiotemporal TF cascades.
a, Tree visualization of the cell lineage leading to corresponding progenitors (colored circles) of defined tissue types. b, Spatiotemporal modules of cell lineage differentiation. Each column represents the development of a tissue progenitor cell lineage (spatial module). Each row represents the classification of the regulation of each progenitor cell lineage into three temporal modules (color gradient). c, Schematic of calculating the similarity in single-cell expression of a given gene. d, Single-cell expression similarity between 30 pairs of TFs known to function in the same pathway24,34,127,132,151,164,165. e, Comparison of expression similarity between genes in the same pathway (n = 30) and between randomly selected gene pairs (n = 30). The boxplot shows the median with IQR and whiskers extending to 1.5× the IQR; outliers are plotted as points. Statistics: Two-tailed Wilcoxon signed-rank test. f, Tree visualization of cellular expression of PHA-4 and CEH-27 in each lineaged cell. The barcode at the bottom indicates the pharyngeal cells. g, Changes in cellular CEH-27 protein expression in pha-4(zu225) embryos. Above showing cellular expression of CEH-27 protein in lineaged cells of wild-type embryos with vertical lines on the bottom indicating cells in which loss or reduction of CEH-27 expression was detected in pha-4(zu225) embryos. Numbers indicate the penetrance of expression changes.
