Extended Data Fig. 5: ELT-1 regulates LIN-32 expression in multiple cell lineages.

a, Single-cell protein expression of ELT-1 and LIN-32. b, Top: Neuronal-specific TFs targeted by ELT-1. Bottom: ELT-1 binding pattern near the lin-32 gene. c, Comparison of single-cell mNeonGreen::LIN-32 presence in all analyzed elt-1(ok1002)/+ and elt-1(ok1002) embryos in lineaged cells. d, Representative micrographs showing loss and gain of mNeonGreen::LIN-32 expression and in cells from various lineages. Similar results were observed in ≥3 embryos. Scale bar, 2 μm. The center Z planes of corresponding cells were identified using mCherry signals and cropped from embryos. Figure organization is as in Fig. 4b. e, Representative micrographs showing loss and gain of cell death in elt-1(ok1002) embryos. Similar results were observed in ≥4 embryos. Scale bar, 2 μm. Programmed cell death was determined by a characteristic sequence of morphological changes in the nucleus of the corresponding cell. The center Z planes of corresponding cells were identified using mCherry signals and cropped from embryos. Figure organization is as in Fig. 4d.