Extended Data Fig. 6: In vitro BURST imaging of ARG-expressing bacteria in plain agarose gel.
From: Ultrasensitive ultrasound imaging of gene expression with signal unmixing
(a-c) Array of ultrasound images of a cross section of rectangular wells containing Nissle E. coli embedded in 1% agarose wells within an agarose phantom. Each image contains a pair of wells, the left well containing RFP-expressing Nissle, the right well containing ARG-expressing Nissle. Rows correspond to cell concentrations, which range over six orders of magnitude. (a) B-mode images. (b) BURST images. (c) BURST+ images. The top edge of each image corresponds to a depth of 17.5 mm, the bottom to a depth of 23 mm. The left edge of each image corresponds to a lateral coordinate of −7 mm, the right to +7 mm. Scalebars: 2 mm. (d) Mean CTR vs log cell concentration for BURST and BURST+ on agarose-embedded cells. N = 12 wells, 4 from each of 3 biological replicates. CTR values represent the mean intensity of the ARG well relative to the mean intensity of the RFP well. Error bars: SEM.
