Extended Data Fig. 8: ARG-expressing Nissle colonies continue to grow and re-express GVs after exposure to BURST+.
From: Ultrasensitive ultrasound imaging of gene expression with signal unmixing
23 hours after initial exposure to BURST+, strong GV-specific BURST+ signal was observed from ARG-expressing Nissle colonies, confirming GV re-expression. Similar results were obtained with two additional plates. (a) Darkfield optical images of the half of the plate exposed to BURST+ after incubation at 30 °C for a total of 15 h (top) and 38 h (bottom). (b) BURST+ composite ultrasound images of the plate after 15 h (top) and 38 h (bottom), with the first collapse frame removed prior to template unmixing to reduce BURST signal area and allow comparison of signal spatial distribution with the optical image. The composite image was formed by taking the maximum of each BURST image plane along the axial dimension and concatenating the resulting rows of pixels to form 2D composite image. Prior to dB scaling, a 3×3 median filter was applied to the composite image, followed by a Gaussian filter with σ = 1. (c) The same BURST+ ultrasound images with all frames included in template unmixing. Scale bars: 10 mm.
