Extended Data Fig. 5: Relationship between ribosome loading and ORF length, tAI or mRNA half-life.
From: Nascent Ribo-Seq measures ribosomal loading time and reveals kinetic impact on ribosome density

(a) Proportion of nascent mRNA in IN or FP after 40 min 4sU from the nRibo-Seq experiment 2 in RAW264.7 macrophages, depicted separately for reads pooled from groups of mRNAs according to ORF length. (b) Proportion of nascent mRNA as in (a), normalized to IN. (c) As in (a) for mRNAs grouped according to tAI. (d) As in (b) for mRNAs grouped according to tAI. (e) As in (a) for mRNAs grouped according to HL. (f) As in (b) for mRNAs grouped according to HL.