Extended Data Fig. 7: Relationship between number of reads and coupling factor.
From: Nascent Ribo-Seq measures ribosomal loading time and reveals kinetic impact on ribosome density
Based on the read numbers in samples of control and LPS-treated RAW264.7 macrophages (15 min, 60 min and 16 h), artificial IN and FP samples were generated with 0.5 to 10 million reads (in steps of 0.5 million). Coupling factors were calculated as in Fig. 4. From 1,000 rounds of simulation, the median, 5th and 95th percentile were determined.
