Fig. 2: Micro-Meta App allows the intuitive and interactive documentation of imaging experiments.
a, Illustrated is the use of the Manage Instrument section of Micro-Meta App to build a graphical record of the hardware components comprising the TIRF Epifluorescence Structured light Microscope (TESM) that was custom built at the Biomedical Imaging Group of UMass Chan Medical School. The resulting diagram allows the collection of all metadata-information sanctioned by Tier 3 (Fig. 1 and Supplementary Information) of the 4DN-BINA-OME model11,13. Specifically, by clicking on icons representing individual hardware components (for example, Objective, Filter Set or Camera), it is possible to record specific information describing each of them (for example, Objective Manufacturer, Model, Magnification and Numerical Aperture (NA)). b, Photograph depicting the TESM microscope illustrated in a. c, Use of the Manage Settings section of Micro-Meta App to build a graphical record of the lightpath (that is, from the illumination source to the detector) used for the acquisition of the 4,6-diamidino-2-phenylindole (DAPI) channel of the image in d. As shown, clicking on icons positioned along the lightpath it is possible to record the acquisition parameters (for example, Camera Bit Depth, Amplification Gain, Binning, etc.) that were used during the acquisition of a given image. d, Representative image obtained using the indicated microscope and settings (a and c).
