Extended Data Fig. 7: Micro-Meta App was utilized at the Centre for Cell Imaging of the University of Liverpool to document a Zeiss Axio Observer Z1 inverted microscope equipped with a LSM 710 confocal scan head and the settings that were applied to the microscope to acquire specific example image-datasets. | Nature Methods

Extended Data Fig. 7: Micro-Meta App was utilized at the Centre for Cell Imaging of the University of Liverpool to document a Zeiss Axio Observer Z1 inverted microscope equipped with a LSM 710 confocal scan head and the settings that were applied to the microscope to acquire specific example image-datasets.

From: Micro-Meta App: an interactive tool for collecting microscopy metadata based on community specifications

Extended Data Fig. 7: Micro-Meta App was utilized at the Centre for Cell Imaging of the University of Liverpool to document a Zeiss Axio Observer Z1 inverted microscope equipped with a LSM 710 confocal scan head and the settings that were applied to the microscope to acquire specific example image-datasets.

Illustrated here is the use of Micro-Meta App for Tier 1 (Minimum Information/Materials & Methods), 4DN-BINA- OME-specified11,12,13 documentation of: (A-B) the Zeiss Axio Observer Z1 inverted microscope equipped with a LSM 710 confocal scan head, owned by the Centre for Cell Imaging (CCI) of the University of Liverpool (Extended Data Fig. 6-Table III); and (C-D) the settings that were applied to the microscope for the acquisition of example published image-datasets73. A) Picture of the indicated microscope. B) Micro-Meta App-generated schematic representation of the indicated microscope. C) Particles composed of superhydrophobic polymer–nanoparticle composite (SPNC) material were analyzed by confocal microscopy to shed light on the differences in composite architecture between different coating polymers. Preparations of SiO2-polydimethylsiloxane (PDMS) coated SPNC (40–75 μm) particles were labelled by staining PDMS with Nile Red. Confocal 3D Z-stacks were acquired using the microscope indicated in Panels A and B. Depicted here is a slice from the middle (24 μm) of a Z-stack depicting a representative particle73. D) Micro-Meta App-generated schematic representation of the lightpath associated with the 561 Channel utilized for the acquisition of the image in Panel C. To demonstrate the functionality and usability of the App and to assess the feasibility of the overall microscopy documentation approach, Micro-Meta App was tested independently at 16 sites (Extended Data Figs. 6–9 and Supplementary Figs. 3–15).

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