Fig. 5: The third axis of the 4DN-BINA-OME Microscopy Metadata Specifications adds further flexibility to minimize the imaging experiment documentation burden. | Nature Methods

Fig. 5: The third axis of the 4DN-BINA-OME Microscopy Metadata Specifications adds further flexibility to minimize the imaging experiment documentation burden.

From: Towards community-driven metadata standards for light microscopy: tiered specifications extending the OME model

Fig. 5

Depicted is an example Venn diagram representing attributes that are required to document the characteristics of an objective lens and are stored in the <Objective> element of the 4DN-BINA-OME Core and the Basic extension. In the schema, objective attributes are color-coded based on their tier level and are subdivided into requirement-level categories based on the following criteria: (1) required (MUST) fields are necessary to validate claims and reproducibility; (2) recommended (SHOULD) fields are prescribed to ensure maximal image quality and sharing value. Color-coding is consistent with that used throughout the manuscript: green, Tier 1; orange, Tier 2; dark blue, Tier 3.

Back to article page