Extended Data Fig. 7: Time- and Vpx-dependent impact of SAMHD1 KO on HIV-1 infection in resting CD4+ T cells. | Nature Methods

Extended Data Fig. 7: Time- and Vpx-dependent impact of SAMHD1 KO on HIV-1 infection in resting CD4+ T cells.

From: Rapid, efficient and activation-neutral gene editing of polyclonal primary human resting CD4+ T cells allows complex functional analyses

Extended Data Fig. 7

SAMHD1 KO cells or NTC-nucleofected reference cells were challenged at the indicated weeks after nucleofection with either HIV-1* GFP without Vpx (left panel) or with Vpx (+ Vpx, right panel) and analyzed for GFP expression on day 3 by flow cytometry. Means ± s.e.m. are shown (n = 4-5). Statistics indicate significance by two-tailed paired t-test. (2 weeks, **P = 0.0035; 4 weeks, *P = 0.0294; 6 weeks*P = 0.0496).

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