Extended Data Fig. 6: Web application allows easy piRNAi transgene design.
From: Reprogramming the piRNA pathway for multiplexed and transgenerational gene silencing in C. elegans
a. Screenshot from www.wormbuilder.org/piRNAi. Simple mode to target a single gene with pre-defined criteria. b. Advanced mode. In the advanced mode, several different piRNA clusters can be re-coded with either custom 20-mers (for example, targeting gfp) or by selecting all 20-mers at a given edit distance mapping to a selected gene isoform. c. Output file with the piRNAi transgene annotated in GenBank format. The sequence is displayed in ‘A plasmid Editor’ (ApE).
