Extended Data Fig. 7: Silencing endogenously gfp-tagged his-72 with piRNAi.
From: Reprogramming the piRNA pathway for multiplexed and transgenerational gene silencing in C. elegans
a. Brightfield and fluorescence images of a strain with an endogenously gfp-tagged his-72 locus. Images show a non-targeting control (mCherry), or sg-piRNAs targeting his-72, gfp, or his-72 + gfp. Images were acquired at 40x magnification using an oil immersion objective. Representative image from more than 50 adult animals imaged. White scale bar = 25 µm. b. We quantified GFP fluorescence in the nucleus of the ‘last’ three oocytes before the spermatheca using ImageJ (NIH). Data are presented as mean values + /- SEM with each data point corresponding to an independently derived transgenic strain. Sample sizes (b) n = 62 (N2), n = 59 (non-targeting), n = 64 (his-72), n = 62 (gfp), n = 62 (his-72 + gfp) fluorescent images from biologically identical transgenic strains across three technical replicates for each condition.
