Extended Data Fig. 1: piRNAi can silence a variety of germline-expressed genes.
From: Reprogramming the piRNA pathway for multiplexed and transgenerational gene silencing in C. elegans
a. Quantification of the number of self-progeny in C. elegans strains with sg-piRNAs targeting the sperm specific genes spe-8 and spe-12. Fertility was assayed from unmated L4 hermaphrodites in a him-5(e1490) mutant background. Control (‘-’) = randomized sg-piRNAs in clusterA. Kruskal–Wallis ANOVA P = 0.0376, Dunn’s multiple comparison, * P = 0.0458, ns = P > 0.99. b. piRNAi against mes-4 and him-5 in wildtype (N2) animals. Transgenic animals were scored for sterile animals and for males (to identify ‘active arrays’). Two-tailed Mann-Whitney, * P = 0.0281 (sterility) and * P = 0.0455 (male frequency). c. piRNAi against dcr-1 and him-5 in wildtype animals (N2). Transgenic L4 stage animals were incubated at 20 °C and 25 °C and their progeny scored for lethality and males. dcr-1 genetic mutants are temperature-sensitive sterile at high temperatures. Two-tailed Mann-Whitney, ns P = 0.1320 and ** P = 0.0065. d. piRNAi against pie-1 and him-5 in the AID::PRG-1 strain maintained on 1 mM auxin (PRG-1 depleted). Larval stage animals from stable, independent transgenic lines were transferred to plates with or without auxin and scored for the number of progeny at 25 °C. Two-tailed Mann-Whitney, ** P = 0.0079. Data are presented as mean values + /- SEM with each data point corresponding to an independently derived transgenic strain. Sample sizes (a) n = 4 (‘-’), n = 4 (spe-8), n = 3 (spe-12), (b) n = 6 (him-5), n = 5 (him-5 + mes-4), (c) n = 6 (all conditions), (d) n = 5 biologically independent transgenic strains.
