Extended Data Fig. 9: Immunohistochemical verification of orexinergic fibers in the somatosensory cortex.
From: A genetically encoded sensor for in vivo imaging of orexin neuropeptides

a, A coronal section of the somatosensory cortex of one mouse showing the laminar spread of OxLight1. Scale bar, 100 µm. b, A high-magnification image (40x) of one coronal section from a brain expressing OxLight-ctr (green) in the somatosensory cortex layer 2/3 (L2/3). The section was stained with Hoechst dye (blue) and with an anti-Orexin-A antibody (white). Scale bar, 50 µm.