Extended Data Fig. 3: GRAND editing achieved efficient and accurate insertion of short fragment.
From: Efficient targeted insertion of large DNA fragments without DNA donors
a, TIDE analysis can measure efficiency of DNA modification within 50 bp range. Insertion of 20 bp or 66 bp with a 53 bp deletion cause -33 bp or +13 bp change, respectively. The editing efficiencies were determined by TIDE analysis. b, Semi-quantitative analysis of 87 bp insertion (with a 53 bp deletion) by agarose gel. c, The efficiencies of accurate insertion of short fragment and imperfect editing were measured by deep sequencing. Mean ± s.d. of n = 3 independent biological replicates.
