Extended Data Fig. 4: SpliZVD calculation example.
From: The SpliZ generalizes ‘percent spliced in’ to reveal regulated splicing at single-cell resolution
The SpliZVD is the projection of the matrix of splicing residuals onto its first eigenvector. The residual matrix’s SVD is used to identify the most variably alternatively spliced sites. The top left shows a gene structure of TPM2, with reads aligning to different junctions above. Each read is assigned a SpliZ residual and SpliZVD residual, the latter of which is based on the first eigenvector (shown below the gene annotations). Ovals representing different cells are colored based on the sign of their SpliZ or SpliZVD values, showing that in this case the SpliZVD is able to distinguish differences in splicing where the SpliZ cannot.
