Fig. 1: Electron-counted MicroED data from milled lamellae of lysozyme.
From: Ab initio phasing macromolecular structures using electron-counted MicroED data
a, A typical lysozyme microcrystal imaged using the FIB. b, A thin, milled lamella from a identified with the TEM. c, MicroED data collected in counting mode using a direct electron detector extending to atomic resolution. The data were summed over a two-degree wedge for display purposes only. A line plot through the box indicated in c is inset, demonstrating the quality of the counting mode data collected showing high peak intensity over background, I/σΙ. d, Simulated detector quantum efficiency (DQE) (e−in/e−out) for this camera operating in counting mode. px, pixel. e, The ab initio phasing strategy in which a small fragment was placed and the initial phases were extended by density modification. The position of the placed fragment and the maps before and after density modification are displayed as indicated to the right.
