Extended Data Fig. 3: Tracking label images using TrackMate.
From: TrackMate 7: integrating state-of-the-art segmentation algorithms into tracking pipelines
a. Mouse hematopoietic stem cells migrating in a hydrogel microwell were automatically segmented using cellpose (Cyto model) implemented in the ZeroCostDL4Mic platform. The resulting label images were automatically tracked using TrackMate (Movie 7). Example raw and label images, as well as local and full cell tracks, are displayed. Yellow squares highlight regions of interest that are magnified. Scale bar = 250 µm. This dataset is available from the Cell Tracking Challenge. b. MCF10DCIS.com cells stably expressing lifeact-RFP and labeled with SiR-DNA were recorded live using a spinning disk confocal microscope. Cells were segmented using cellpose (Cyto model) implemented in the ZeroCostDL4Mic platform. The resulting label images were tracked using TrackMate (Movie 8). Example raw and label images, as well as local and full cell tracks, are displayed. Yellow squares highlight regions of interest that are magnified. Scale bar = 250 µm.
