Extended Data Fig. 7: etSTED experiments in HeLa cells with widefield imaging of dynamin (dynamin1-EGFP) and 3D STED timelapse imaging of membrane dynamics (cholesterol-KK114) during endocytosis.

a, Widefield images leading up to the triggering frame (last image), showing the slow rise in fluorescence signal signifying recruitment of dynamin at the site. b, Representative frames from 5.9 Hz 3D STED timelapses of XZ cross-sections across the detected event, showing various membrane dynamics during the endocytosis events taking place. Arrows marks the scanned section in X (a). N = 53 events, N = 22 cells. Same scales apply to all frames of the timelapses. Asterisks (*) indicate deconvolved frames, and applies to all following frames in the same timelapse. Scale bars, 2 μm (a), 250 nm (b).