Extended Data Fig. 8: etSTED experiments in HeLa cells with widefield imaging of CD63-pHluorin and 3D STED timelapse imaging of membrane dynamics (cholesterol-KK114) during exocytosis. | Nature Methods

Extended Data Fig. 8: etSTED experiments in HeLa cells with widefield imaging of CD63-pHluorin and 3D STED timelapse imaging of membrane dynamics (cholesterol-KK114) during exocytosis.

From: Event-triggered STED imaging

Extended Data Fig. 8

a, Widefield images leading up to the triggering frame (last image), showing the rapidly appearing (frame-to-frame) fluorescence signal signifying a local pH neutralization at the site. b, Representative frames from 11 Hz 3D STED timelapses of XZ cross-sections across the detected event, showing various membrane dynamics during the exocytosis events taking place. Arrows marks the scanned section in X (a). N = 232 events, N = 29 cells. Same scales apply to all frames of the timelapses. Asterisks (*) indicate deconvolved frames, and applies to all following frames in the same timelapse. Scale bars, 2 μm (a), 250 nm (b).

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