Extended Data Fig. 6: Measured integrated Ca2+ fluorescence signals and estimated significance for TCR55 negative controls.
(a) Left: Integrated Ca2+ signals for each positive cell (light grey markers) and the mean integrated Ca2+ signal (light grey square) at 34 °C. Right: Estimated p-value vs. log2-transformed mean fold change for TCR55-tranduced T cells interacting with 21 different peptide sequences at 34 °C. Grey box indicates Bonferroni-corrected p-value at a significance of 0.05 (p = 0.0024). Correspondence between thermo-responsive bead spectral code and displayed peptide sequence is shown at right. n = 1320 cells in total. (b) Left: Integrated Ca2+ signals for each positive cell (light grey markers) and the mean integrated Ca2+ signal (light grey square) at 37 °C. Right: Estimated p-value vs. log2-transformed mean fold change for TCR55-tranduced T cells interacting with 21 different peptide sequences at 37 °C. Grey box indicates Bonferroni-corrected p-value at a significance of 0.05 (p = 0.0024). Correspondence between thermo-responsive bead spectral code and displayed peptide sequence is shown at right. n = 845 cells in total. The box width in (a) and (b) defines the interquartile range (IQR) and whiskers extend to 1.5 × IQR in either direction. p-values were calculated via two-sided bootstrapping as detailed in Methods. (c) Integrated Ca2+ signals for all positive cells (dark grey markers) at 34 °C, 37 °C and with expansion force applied (bars and error bars indicate mean and standard deviation, respectively; positive cell numbers and percentages are indicated above each bar.
