Extended Data Fig. 7: Measured integrated Ca2+ fluorescence signals and estimated significance for TCR55 interacting with either: (1) PEG-DA beads (which do not change size upon a small temperature change) bearing the stimulatory peptide VPLTEDALL; (2) thermo-responsive NIPAM beads bearing the non-stimulatory peptide VPIVEDSFL; and (3) thermo-responsive NIPAM beads bearing the stimulatory peptide VPLTEDALL.
(a) Results at 34 °C alone, (b) results at 37 °C alone, and (c) results for heating to 37 °C and then cooling to 34 °C. Each panel shows: (left) integrated Ca2+ signals for each positive cell (light grey markers) and the mean integrated Ca2+ signal (light grey square) and (right) estimated p-value vs. log2-transformed mean fold change for TCR55-tranduced T cells interacting with each peptide-bead combination. Positive cell percentages are indicated above each bar. Grey box indicates Bonferroni-corrected p-value at a significance of 0.05 (p = 0.0167). Correspondence between embedded spectral code and displayed peptide sequence is shown at right. In all box plots, the box width defines the interquartile range (IQR) and whiskers extend to 1.5 × IQR in either direction. p-values were calculated via two-sided bootstrapping as detailed in Methods.
