Extended Data Fig. 9: Force multiplex for two TCR55 replicates.
(a) Measured integrated Ca2+ fluorescence signals for each positive cell (light grey markers) and the mean integrated Ca2+ signal (light grey square) for two TCR55 replicates interacting with 4 different peptides at three force ramps for Replicate 1 (top) and Replicate 2 (bottom). The box width defines the interquartile range (IQR) and whiskers extend to 1.5 × IQR in either direction. n = 1498 cells and 1256 cells for rep. #1 and rep. #2, respectively. (b) Correlation plot of the means of integrated Ca2+ signals between two replicates. Dashed black line indicates the line with a slope equal to 1; red dashed line indicates a linear regression. Error bars indicate SEM. (c) Integrated Ca2+ signal as a function of applied force for 4 peptides for Replicate 2. Zero force data is from a TCR55 control experiment where the temperature was maintained at 34 °C; error bars indicate SEM. n = 1256 cells for force ramps experiments and 1320 cells at zero force ramp. Error bars indicate SEM.
