Fig. 5: High-resolution structural determination of small macromolecules by single-particle cryo-EM.
From: Uniform thin ice on ultraflat graphene for high-resolution cryo-EM
a,d,g, Typical cryo-EM micrographs of hemoglobin (a), α-fetoprotein (d) and streptavidin (g) supported by UFG. The insets show representative 2D class averages of the particle images Scale bars, 20 nm. b,e,h, The density maps of hemoglobin at 3.5 Å resolution, with individual monomers indicated by different colors (b), α-fetoprotein at 2.6 Å resolution, with individual domains indicated by different colors (e), and streptavidin at 2.2 Å resolution, with individual monomers indicated by different colors (h). c,f,i, Selected densities from the hemoglobin map (c) and α-fetoprotein map (f) with the corresponding atomic models docked; and the density of several amino acid residues extracted from the 2.2 Å streptavidin map with the corresponding atomic models of the residues (i).
