Extended Data Fig. 1: Ligand binding site of FKBP and directed evolution results of CapN.
From: A general method for chemogenetic control of peptide function
Related to main Fig. 1. a, Crystal structure of FKBP12 (PDB:1FAP). The hydrophobic residues around the ligand binding site are shown in yellow and stick representation. b, Sequences of forty clones from the post 4th round CapN library as shown in main Fig. 1e. Twenty-three distinct sequences were identified. Clone #1 is the final CapN used for the rest of this study. c, FACS analysis of the most enriched eight clones, corresponding to clones #1-#8 shown in b. Values are median HA intensity of FLAG-positive cells (Q2 + Q4). All eight clones showed similar results. This experiment was performed once.
