Extended Data Fig. 4: ECM protein expressions in 3D-oBRB. | Nature Methods

Extended Data Fig. 4: ECM protein expressions in 3D-oBRB.

From: Bioprinted 3D outer retina barrier uncovers RPE-dependent choroidal phenotype in advanced macular degeneration

Extended Data Fig. 4

a-f, 3D reconstructed images of tissues immunostained for CD31 (magenta, a-f), nuclei (blue, a-f), LAMININ (LMN, green a, b), ELASTIN (ELN, green, c, d), COLLAGEN IV (COL IV, green, e, f), viewed at the subRPE level (a, c, e) and choriocapillaris level (b, d, f). N = 3. g, cross section of 3D-oBRB immunostained for Bruch’s membrane proteins LAMININ (yellow) and COLLAGEN IV (COL IV, magenta). Nuclei stained with DAPI (cyan). N = 3. Scale bar, 10μm.

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