Extended Data Fig. 2: Singleplex library complexity.
From: Multiplexed transcriptome discovery of RNA-binding protein binding sites by antibody-barcode eCLIP
The number of unique molecules was estimated as a function of sequencing depth. After randomly sampling uniquely mapped reads from ABC, eCLIP, and iCLIP, we plot the number of uniquely mapped reads vs the number of usable reads. A) RBFOX2 (HEK293XT) B) SLBP (K562). C) qPCR amplification plot of RBFOX2 ABC with and without UV crosslinking.
