Extended Data Fig. 6: Analysis of tail composition using Nano3P-seq in in vivo samples.
(a) Overall nucleotide composition in mRNA tails at 3 time points during the zebrafish MZT (2, 4 and 6 hpf, shown in blue, green and red respectively) and control that includes sequin R1 and R2 groups of RNAs (gray). P-values have been computed using Kruskal-Wallis test. (n = 3 biological replicates, data are presented as mean values+/− standard error). (b) Probability of base composition (A: green, G: orange, C: blue and U: red) per position in the last 20 nucleotide of the mRNA tails at 3 time points during the zebrafish MZT (2, 4 and 6 hpf). (c) IGV snapshots of reads mapping to zebrafish ppt2a.4 mRNA (left panel). In the right panel, zoomed images of 3′ ends of individual reads with different terminal bases (all reads: top, Term-G reads: bottom) are shown. (d) PolyA tail length estimation distributions of mRNA reads belonging to groups classified based on their polyA tail base composition in mouse. (e) PolyA tail length estimation distributions of mRNA reads belonging to groups classified based on their polyA tail base composition in yeast. (p > 0.05:ns, p ≤ 0.05:*, p ≤ 0.01:**, p ≤ 0.001:***, p ≤ 0.0001:****).
