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Long-read sequencing in the era of epigenomics and epitranscriptomics

Nature Methods volume 20, pages 25–29 (2023)Cite this article

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As long-read sequencing technologies continue to advance, the possibility of obtaining maps of DNA and RNA modifications at single-molecule resolution has become a reality. Here we highlight the opportunities and challenges posed by the use of long-read sequencing technologies to study epigenetic and epitranscriptomic marks and how this will affect the way in which we approach the study of health and disease states.

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Fig. 1: List of DNA and RNA modifications, and sequencing-based methods used to detect them.
Fig. 2: Overview of methods for detecting DNA and RNA modifications using long-read sequencing.

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Acknowledgements

M.C.L. is supported by an FPI Severo-Ochoa fellowship from the Spanish Ministry of Economy, Industry, and Competitiveness (MEIC). This work was supported by funds from MEIC (PID2021-128193NB-100 to E.M.N.) and the European Research Council (ERC-StG-2021 No 101042103 to E.M.N.). We acknowledge the support of the MEIC to the EMBL partnership, Centro de Excelencia Severo Ochoa, and CERCA Programme/Generalitat de Catalunya.

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Authors and Affiliations

  1. Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain

    Morghan C. Lucas & Eva Maria Novoa

  2. Universitat Pompeu Fabra (UPF), Barcelona, Spain

    Morghan C. Lucas & Eva Maria Novoa

Authors
  1. Morghan C. Lucas
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  2. Eva Maria Novoa
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Contributions

M.C.L. and E.M.N. cowrote the manuscript. M.C.L. composed the figures.

Corresponding author

Correspondence to Eva Maria Novoa.

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Competing interests

E.M.N. has received travel and accommodation expenses to speak at Oxford Nanopore Technologies conferences, and is a member of the Scientific Advisory Board of IMMAGINA Biotech. M.C.L. has received an Oxford Nanopore Technologies travel bursary.

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Lucas, M.C., Novoa, E.M. Long-read sequencing in the era of epigenomics and epitranscriptomics. Nat Methods 20, 25–29 (2023). https://doi.org/10.1038/s41592-022-01724-8

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