Fig. 1: Design and principle of ELI-TriScope.
From: ELI trifocal microscope: a precise system to prepare target cryo-lamellae for in situ cryo-ET study
a, Schematic diagram and design drawing of ELI-TriScope in its operational mode. Each part of the system is labeled and described. IB, ion beam; LB, light beam; EB, electron beam. b, Working principle of ELI-TriScope. The ion beam and light beam are simultaneously focused on the area of interest of the cryo-specimen. The reflected light image recorded by the camera displays the cell body, and the target signal in the fluorescence image is used to navigate cryo-FIB milling. LM, light microscope.
