Extended Data Fig. 8: LSBM allows Brillouin time-lapse imaging of developing mouse embryos over ~2 days.

(a) Timeline of mouse embryo development from the one cell stage to late blastocyst. The dashed rectangle encloses the developmental window that was imaged. (b) Exemplary Brillouin volumes acquired in the orthogonal geometry (top) and SPIM volumes (bottom) of a single mouse embryo at the beginning (left) and at end (46h45m after the first timepoint) of the time-lapse (right). The acquisition time for one volume in the Brillouin modality was ~11–17 min, the time interval between volumes is between 77 to 92 minutes. Representative result from n = 4 embryos. Also see Supplementary Videos 5, 6. (c) Top: SPIM volume (maximum intensity projection -MIP) of an embryo that underwent Brillouin time-lapse imaging, taken at the end of the time-lapse (representative image out of 4 embryos). Bottom: SPIM image (MIP) of a control embryo (taken at the same time as the embryo in top panel), that was in the same imaging chamber but not imaged by Brillouin or SPIM, showing qualitatively similar morphology (representative image out of 9 embryos). (d) MIPs through the volume where the outer trophectoderm cells are CDX2-positive (green), a marker for trophectoderm cell fate. The inner cell mass are SOX2-positive (red) and CDX2-negative, indicating proper epiblast fate in the ICM at late blastocyst stage. The number of cells in the ICM is 26, consistent with the reported values in literature [15]. Representative results from n = 3 embryos.