Extended Data Fig. 1: Optical and mechanical design of the LSBM.

(a) Conceptual schematic of the optical layout including both the fluorescence and Brillouin imaging modalities. The ASE filter and spectrometer are further detailed in Extended Data Figs. 2 and 3, respectively. (b) Optical path for the Brillouin illumination and detection in the orthogonal (top) and epi line (bottom) configuration. Note that switching between these configurations can be done by flipping the mirror after the tunable lens, adding/removing the PBS (mounted on a magnetic base to ease this operation) and rotating the half and quarter waveplates appropriately. (c) 3D rendering of the mechanical design. The objectives are arranged in an inverted 90 deg V-configuration; light is delivered to (and collected from) the objectives by means of two mirrors that preserve image orientation. (d) Enlarged view of the immersion chamber, showing the heating element inset used for temperature control. See Methods for further details.