Extended Data Fig. 3: Optical design of the LSBM spectrometer. | Nature Methods

Extended Data Fig. 3: Optical design of the LSBM spectrometer.

From: High-resolution line-scan Brillouin microscopy for live imaging of mechanical properties during embryo development

Extended Data Fig. 3

(a) Orthogonal views of the optical components in the LSBM spectrometer with relevant distances; top shows the plane determined by the illumination line and the optical axis of the detection objective, where the optics relay to the VIPA and appropriately magnify the back focal aperture of the objective; bottom shows the plane perpendicular to the illumination line, where the optics relay to the VIPA and appropriately magnify the image plane. (b) Details of the relay shown in panel a.

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