Extended Data Fig. 4: Optical characterization and precision measurement of the LSBM spectrometer.
Orthogonal views of a 0.3um bead (Tetraspeck, Thermo Fischer) to characterize the optical resolution in the orthogonal (a) and epi line (b) geometries. (c) Spatial calibration (µm to pixel) acquired by translating a bead, with steps of 10 µm, along the x direction and determining its position on the camera. (d) Precision vs. illumination energy for the orthogonal (red) and epi (blue) line geometries. The shaded red region indicates the typical imaging conditions used in the experiments. To generate the precision plot for the O-LSBM (E-LSBM), 441 (441) spectrometer images were acquired from water, each containing more than 200 spectra (one for each spatial position); the data points (circles) represent the precision, calculated as the S.D. of the Brillouin shift extracted from all the spectra selected from 61 (31) at the center of the FOV – that is 26901 (13671) spectra in total; the error bars represent the S.D. of the precision calculated as mentioned before but separately for each point along the FOV– that is the S.D. of 61 (31) values. See Methods for details.
