Extended Data Fig. 9: Concurrent measurement of the release of cytokine and cytotoxic molecule by SCA.
From: Time-resolved assessment of single-cell protein secretion by sequencing
(A) HyNic-4FB chemistry-based covalent conjugation of anti-CD45 and anti-granzyme B (GrB) to form bispecific antibody. (B) T cells modified with CD45-GrB bispecific antibodies are able to capture extracellular GrB molecules with high efficiency. (C, D) Secretion capture of GrB, IFN-γ and TNF-α over a period of 10 hours in CD8+ T cells (C) and CD8− T cells (D) after stimulation with anti-CD3/CD28 Dynabeads (beads-to-cell ratio = 1:1) in 96-well U-bottom plate.
